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KMID : 1024520140230050895
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Journal of the Environmental Sciences
2014 Volume.23 No. 5 p.895 ~ p.902
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Static Culture Condition for Production of Bacterial Cellulose, Environment-Friendly Functional Material, by Acetic Acid Bacteria
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Cho Kwang-Sik
Lee Sang-Mi
Jeong Seong-Yun
Park Geun-Tae
Lee Hee-Sup
Hwang Dae-Yean
Jung Young-Jin
Son Hong-Joo
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Abstract
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Bacterial cellulose (BC) has played important role as new functional material for food industry and industrial products
based on its unique properties. The interest in BC from static cultures has increased steadily in recent years because of its potential for use in medicine and cosmetics. In this study, we investigated culture condition for BC production by Acetobacter sp. F15 in static culture. The strain F15, which was isolated from decayed fruit, was selected on the basis of BC thickness. The optimal medium compositions for BC production were glucose 7%, soytone 12%, K2HPO4 0.2%, NaH2PO4ㆍ2H2O 0.2%, lactic acid 0.05% and ethanol 0.3%, respectively. The strain F15 was able to produce BC at 26℃-36℃ with a maximum at 32℃. BC production occurred at pH 4.5-8 with a maximum at pH 6.5. Under these conditions, a maximum BC thickness of 12.15mm was achieved after 9 days of cultivation; this value was about 2.3-fold higher than the thickness in basic medium.
Scanning electron micrographs showed that BC from the optimal medium was more compact than plant cellulose and was
reticulated structure consisting of ultrafine cellulose fibrils. BC from the optimal medium was found to be of cellulose type I,
the same as typical native cellulose.
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KEYWORD
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Acetobacter, Bacterial cellulose, Static culture
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